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KMID : 0613820130230111351
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Journal of Life Science
2013 Volume.23 No. 11 p.1351 ~ p.1359
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Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells
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Lee Sun-Min
Kim Young-Sook
Jang Wook-Jin
Rakib Abdur Md.
Oh Tae-Woo
Kim Boh-Hyun
Kim So-Young
Kim Jeong-Ok
Ha Yeong-Lae
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Abstract
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The anti-proliferative activity of β-ionone was investigated on human non-small lung cancer A-549 cells (designated A-549 cells). A-549 cells were treated with various concentrations of β-ionone (1, 5, 10, and 15 μM) for two, four, and six days. Biochemical markers related to the growth inhibition of A-549 cells by β-ionone were measured at the second day of incubation. β-Ionone inhibited the growth of A-549 cells by dose-and time-dependent manners, resulting in an IC_50 of 5.0 μg/ml at the second day of incubation. β-Ionone induced apoptosis by a dose-dependent manner. β-Ionone increased levels of p53, p21, and Bax proteins, but suppressed expression of the Bcl-2 protein. Similarly, β-ionone enhanced cytochrome c release from the mitochondria to the cytosol, and induced activation of caspase-9 and -3. Additionally, β-ion-one reduced cPLA₂ and COX-2 protein levels. These results suggest that the β-ionone inhibits the proliferation of A-549 cells through reciprocal regulation of Bax and Bcl-2 gene expression and suppression of cPLA₂ and COX-2 protein expressions.
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KEYWORD
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β-Ionone, human non small lung cancer A-549 cells, apoptosis, cPLA₂, Cox-2
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